A comprehensive study from the micro- to the nanometric scale: Evaluation of chilling injury in tomato fruit (Solanum lycopersicum).

Tomato fruit is susceptible to chilling injury (CI) during its postharvest handling at low temperature. The symptoms caused by this physiological disorder have been commonly evaluated by visual inspection at a macro-observation scale on fruit surface; however, the structure at deeper scales is also affected by CI. This work aimed to propose a descriptive model of the CI development in tomato tissue under the micro-scale, micro-nano-scale and nano-scale approaches using fractal analysis. For that, quality and fractal parameters were determined. In this sense, light microscopy, Environmental Scanning Electron Microscopy (ESEM) and Atomic Force Microscopy (AFM) were applied to analyse micro-, micro-nano- and nano-scales, respectively. Results showed that the morphology of tomato tissue at the micro-scale level was properly described by the multifractal behaviour. Also, generalised fractal dimension (Dq=0) and texture fractal dimension (FD) of CI-damaged pericarp and cuticle were higher (1.659, 1.601 and 1.746, respectively) in comparison to non-chilled samples (1.606, 1.578 and 1.644, respectively); however, FD was unsuitable to detect morphological changes at the nano-scale. On the other hand, lacunarity represented an appropriate fractal parameter to detect CI symptoms at the nano-scale due to differences observed between damaged and regular ripe tissue (0.044 and 0.025, respectively). The proposed multi-scale approach could improve the understanding of CI as a complex disorder to the development of novel techniques to avoid this postharvest issue at different observation scales.

Impact of SARS-CoV-2 variants on mobility and air pollution in the United Kingdom.

During the two years following the first case of COVID-19 in the United Kingdom, cycles of social restrictions were imposed to control the spread of the virus. These measures curtailed social contact and halted commercial and recreational activities affecting levels of air pollutants. As society adapted, restrictions eased and pollution gradually returned to baseline levels. However, resurgence in COVID-19 cases from new variants created a protracted and challenging path back to 'normality'. In this study, we retrospectively look back at the two years of COVID-19 and its prevalent variants, and examine the government response and its impact on mobility and air pollution. Results from a peak detection algorithm show peak events in mobility and COVID-19 deaths during variants periods decreased significantly from the wildtype COVID-19, despite the high contagiousness of these variants. Pollution levels remained below baseline with periods of significant increase for O3, while NO2 levels remained depleted, likely as a result of reduced traffic congestion as home office schemes have been maintained. Our findings suggest mobility and pollution return to baseline levels as immunity to COVID-19 increases.